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Prototype amperometric biosensor for sialic acid determination.

Sayed A M Marzouk1, S S Ashraf, Khawla A Al Tayyari

  • 1Department of Chemistry, Faculty of Science, United Arab Emirates University, Al-Ain, PO Box 17551, United Arab Emirates. sayedm@uaeu.ac.ae

Analytical Chemistry
|February 15, 2007
PubMed
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This study presents a novel amperometric biosensor for detecting free sialic acid (SA). The developed biosensor demonstrates high sensitivity and reproducibility, enabling applications in biological and pharmaceutical analysis.

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Biochemistry

Background:

  • Sialic acid (SA) is a crucial biomarker in various biological processes and diseases.
  • Accurate and sensitive detection of SA is vital for clinical diagnostics and pharmaceutical research.
  • Existing methods for SA detection can be complex and time-consuming.

Purpose of the Study:

  • To develop and characterize a novel amperometric biosensor for the detection of free sialic acid (SA).
  • To evaluate the performance and applicability of the SA biosensor in biological and pharmaceutical samples.
  • To expand the biosensor's capability to detect bound sialic acid.

Main Methods:

  • Co-immobilization of N-acetylneuraminic acid aldolase and pyruvate oxidase enzymes on a platinum disk electrode.

Related Experiment Videos

  • Detection of hydrogen peroxide produced by enzymatic reactions via anodic amperometry.
  • Electropolymeric coating of the electrode surface to enhance selectivity.
  • Optimization of enzyme layer composition and operating conditions (pH 7.2, 37°C).

    • Main Results:

    • The SA biosensor exhibited a limit of detection of 10 µM with linearity up to 3.5 mM (r = 0.9987).
    • The biosensor maintained 85% sensitivity after 8 days with excellent reproducibility (CV = 2.3%).
    • The inclusion of sialidase enabled the detection of bound sialic acid, broadening its application scope.

    Conclusions:

    • The developed amperometric biosensor is a sensitive, selective, and reproducible tool for free and bound sialic acid determination.
    • The biosensor shows promise for bioanalytical applications, including tumor marker screening and therapy monitoring.
    • This biosensor offers a valuable alternative for SA analysis in biological and pharmaceutical contexts.