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T-cell activation in occupational asthma and rhinitis
E Mamessier1, F Milhe, C Guillot
1Université de la Méditerranée, Marseille, France.
Allergy
|February 15, 2007
Summary
Occupational asthma and rhinitis show distinct T-cell activation profiles. Specific inhalation tests reveal a gradient from tolerance in exposed nonallergic subjects to inflammation in occupational asthma.
Area of Science:
- Immunology
- Allergy Research
- Occupational Health
Background:
- Allergic asthma and rhinitis are often linked to Th2 activation, but Th1 activation and regulatory T (Treg) cell defects are increasingly recognized.
- The T-cell activation profile in occupational asthma (OA) and occupational rhinitis (OR) remains largely unknown.
Purpose of the Study:
- To characterize T-cell activation following a specific inhalation test (SIT) in individuals with OA and OR.
- To compare T-cell responses in OA, OR, exposed nonallergic (ENA) subjects, and healthy volunteers.
Main Methods:
- Included 21 OA, 10 OR, 10 ENA, and 14 healthy subjects.
- Performed SIT with the causative agent, collecting blood and induced sputum before and after.
- Analyzed T-cell activation markers (CD69, CD25), IL-13, and IFN-gamma via flow cytometry and ELISA; identified Treg cells.
Main Results:
- Baseline IFN-gamma was lower in OA and OR patients.
- SIT increased Th1 and Th2 cells and decreased Treg cells in OA patients.
- OR showed an attenuated response with increased IL-13+ T cells; ENA subjects displayed Th2 activation with increased Treg cells and decreased Th1 cells.
Conclusions:
- Demonstrated a gradient of T-cell activation: tolerance in ENA subjects, intermediate in OR, and inflammatory in OA.
- Highlights distinct immune responses in occupational airway diseases.
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