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Published on: September 11, 2015
In vitro osteogenesis on a microstructured titanium surface with additional submicron-scale topography.
Humberto Osvaldo Schwartz Fo1, Arthur Belém Novaes, Larissa Moreira Spinola de Castro
1Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Clinical Oral Implants Research
|February 15, 2007
Summary
The Plus titanium surface showed early matrix mineralization but not enhanced bone formation. The Deep Profile Surface (DPS) resulted in greater mineralized tissue compared to Plus and machined surfaces in vitro.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Orthopedic Research
Background:
- Titanium's surface topography influences bone regeneration.
- Understanding osteogenesis on microstructured titanium is crucial for dental and orthopedic implants.
Purpose of the Study:
- To compare in vitro osteogenesis on three titanium surface topographies: Plus, Deep Profile Surface (DPS), and machined.
- To evaluate cell adhesion, proliferation, mineralization, and bone-like tissue formation.
Main Methods:
- Calvaria-derived osteogenic cultures were used.
- Assessed parameters included cell adhesion, growth, viability, alkaline phosphatase (ALP) activity, protein content, and immunolocalization of bone markers (fibronectin, BSP, OPN).
- Bone-like tissue formation and mineralization were analyzed at days 11 and 17.
Main Results:
- No significant differences in cell adhesion, growth, viability, ALP activity, or total protein content were observed across surfaces.
- The Plus surface showed early, localized calcified matrix at day 11, which enlarged by day 17 but lacked mature osteoblastic markers.
- The DPS surface exhibited significantly larger total mineralized areas by day 17 compared to Plus and machined surfaces.
- Increased apoptotic bodies were noted on the Plus surface associated with mineralized nodules.
Conclusions:
- The Plus surface promotes an early, distinct pattern of matrix mineralization.
- This early mineralization on the Plus surface does not necessarily lead to superior overall bone-like tissue formation in vitro.
- The DPS surface demonstrated a greater capacity for in vitro bone-like tissue accumulation compared to the Plus and machined surfaces.
