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Multiparallel microfluidic high-performance liquid chromatography for high-throughput normal-phase chiral analysis.

Peter Sajonz1, Wes Schafer, Xiaoyi Gong

  • 1Separation & Analysis Technologies, Merck and Co., Inc., Rahway, NJ 07065, USA. peter_sajonz@merck.com

Journal of Chromatography. A
|February 16, 2007
PubMed
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The Eksigent Express 800 microfluidic HPLC instrument enables rapid, multiparallel normal-phase chiral analysis for pharmaceutical research. This high-throughput system significantly accelerates enantiopurity assessment and catalyst screening compared to traditional methods.

Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Science
  • Chemical Engineering

Background:

  • High-throughput screening is crucial for pharmaceutical process research.
  • Conventional chiral analysis methods are time-consuming.
  • Microfluidic systems offer potential for accelerated analytical workflows.

Purpose of the Study:

  • To evaluate the Eksigent Express 800 microfluidic HPLC instrument for multiparallel normal-phase chiral analysis.
  • To assess its suitability for high-throughput pharmaceutical process research.
  • To demonstrate its application in enantiopurity assessment and catalyst screening.

Main Methods:

  • Utilized the Eksigent Express 800 eight-channel HPLC instrument.
  • Performed multiparallel normal-phase chiral analysis using 96-well microplates.

Related Experiment Videos

  • Conducted method development including gradient screening and optimization for asymmetric hydrogenation catalyst screening.

    • Main Results:

    • Achieved rapid (<2 h) and accurate enantiopurity assessment of benzoin and dihydrobenzoin enantiomers.
    • Successfully supported high-throughput catalyst screening for asymmetric hydrogenation within one working day.
    • Demonstrated a significant improvement over conventional multi-day analysis techniques.

    Conclusions:

    • The Eksigent Express 800 is a suitable instrument for high-throughput multiparallel normal-phase chiral analysis in pharmaceutical research.
    • The system offers substantial time savings for enantiopurity determination and catalyst screening.
    • Microfluidic HPLC provides a powerful tool for accelerating drug discovery and development processes.