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Updated: Jul 16, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption
Tony Cano1, Natalie Offringa, Richard C Willson
1Department of Chemical Engineering, University of Houston, 4800 Calhoun Ave., Houston, TX 77204-4004, USA.
Abstract:
Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b(5), E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.
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