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Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
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Related Experiment Video

Updated: Jul 16, 2026

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
09:15

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Published on: January 21, 2020

Curcumin induced suicidal erythrocyte death.

Peter J Bentzen1, Elisabeth Lang, Florian Lang

  • 1Department of Physiology, University of Tübingen, Tübingen, Germany.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|February 21, 2007
PubMed
Summary

Curcumin, a natural compound, triggers eryptosis, or suicidal erythrocyte death, by increasing cell shrinkage and phosphatidylserine exposure. This may impact the lifespan of red blood cells.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Hematology

Background:

  • Curcumin exhibits anti-inflammatory and antitumor properties.
  • Curcumin induces apoptosis in nucleated cells.
  • Eryptosis is characterized by erythrocyte shrinkage and phosphatidylserine exposure, leading to clearance from circulation.

Purpose of the Study:

  • To investigate Curcumin's potential to induce eryptosis in erythrocytes.
  • To determine the mechanisms underlying Curcumin-induced eryptosis.

Main Methods:

  • Erythrocytes were exposed to Curcumin (1 microM).
  • Annexin V binding was measured to assess phosphatidylserine exposure.
  • Forward scatter in FACS analysis quantified cell volume.
  • Cytosolic Ca(2+) activity was measured using Fluo3 fluorescence.
  • Ceramide formation was assessed via immunofluorescence.

Main Results:

  • Curcumin exposure increased annexin V binding and decreased forward scatter, indicating cell shrinkage and phosphatidylserine exposure.
  • Curcumin elevated cytosolic Ca(2+) activity and promoted ceramide formation.
  • Curcumin enhanced the effects of hypertonic shock, chloride removal, and glucose depletion on annexin V binding.
  • Curcumin reversed the shrinking effect of glucose withdrawal.

Conclusions:

  • Curcumin exhibits a proeryptotic effect on erythrocytes.
  • Curcumin-induced eryptosis involves increased cytosolic Ca(2+) and ceramide formation.
  • This action of Curcumin may influence the lifespan of circulating erythrocytes.