Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PathogenMip assay: a multiplex pathogen detection assay.

Michael S Akhras1, Sreedevi Thiyagarajan, Andrea C Villablanca

  • 1Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America.

Plos One
|February 22, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive methods to monitor dynamic single-cell events.

Trends in cell biology·2026
Same author

3D ECM-inflammation model on a microfluidic chip for neutrophil transmigration from whole blood investigations.

Lab on a chip·2026
Same author

Virus Genome Sequences in the Blood of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients.

medRxiv : the preprint server for health sciences·2025
Same author

Benchmarking long-read assembly tools and preprocessing strategies for bacterial genomes: A case study on <i>E. coli</i> DH5α.

Biotechnology reports (Amsterdam, Netherlands)·2025
Same author

Dynamic and precise electromagnetic levitation of single cells.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Light-orchestrated multi-step solid-phase picodroplet reactors.

bioRxiv : the preprint server for biology·2025

The novel PathogenMip Assay offers a cost-effective method for pathogen detection using highly discriminating probes. This assay accurately genotypes human papillomavirus (HPV), showing potential for broad microbial identification.

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • The Molecular Inversion Probe (MIP) assay is established for human SNP detection.
  • Pathogen detection requires precise and accessible genotyping methods.

Purpose of the Study:

  • To introduce the PathogenMip Assay, a comprehensive protocol for pathogen detection.
  • To develop a cost-effective method for producing highly discriminating probes for genotyping.

Main Methods:

  • Developed a novel, ligase-based protocol for probe production.
  • Targeted 24 human papillomavirus (HPV) genotypes associated with cervical cancer.
  • Validated the assay using pyrosequencing, microarray detection, and conventional sequencing.

Main Results:

Related Experiment Videos

  • Achieved 100% agreement with conventional sequencing for HPV genotyping in tumor DNA.
  • Demonstrated high sensitivity and selectivity using HPV plasmids.
  • Successfully genotyped clinically relevant HPV genotypes.

Conclusions:

  • The PathogenMip Assay provides a widely accessible protocol for pathogen genotyping.
  • The assay is experimentally validated and shows potential for detecting and characterizing diverse microbes.