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DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
Published on: March 20, 2021
A water-soluble, octacationic zinc phthalocyanine as molecular probe for nucleic acid secondary structure
An-Ming Zhang1, Jing Huang, Xiao-Cheng Weng
1College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan, PR China.
Abstract:
The interaction between CT-DNA and the zinc phthalocyanine ZnPc (1) was studied by UV/VIS and fluorescence titration, as well as by thermal denaturation. ZnPc was found to strongly bind to CT-DNA (K(app)=7.35 x 10(5) M(-1)) in a non-intercalative mode. The photosensitized cleavage of pBR322 DNA was found to efficiently proceed via singlet-oxygen ((1)O(2)) production. Further, ZnPc (1) caused site-specific scission of guanine (G) bases around the bulge of the hairpin oligonucleotides OD1-OD3, as clearly shown by gel-electrophoresis experiments.
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