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Related Experiment Videos

Physicochemical studies on pepsin-CTAB interaction: energetics and structural changes.

Tanushree Chakraborty1, Indranil Chakraborty, Satya P Moulik

  • 1Centre for Surface Science, Department of Chemistry, Jadavpur University, Kolkata 700032, India.

The Journal of Physical Chemistry. B
|February 22, 2007
PubMed
Summary
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The interaction between pepsin and cetyltrimethylammonium bromide (CTAB) forms complexes and micelles, altering pepsin

Area of Science:

  • Biochemistry
  • Physical Chemistry
  • Materials Science

Background:

  • Pepsin, a key digestive enzyme, interacts with surfactants.
  • Cetyltrimethylammonium bromide (CTAB) is a cationic surfactant known for micelle formation.

Purpose of the Study:

  • To investigate the physicochemical interactions between pepsin and CTAB.
  • To characterize the resulting complexes, aggregates, and micelles.
  • To understand the effect of CTAB on pepsin's structure and interfacial properties.

Main Methods:

  • Surface tension measurements (tensiometry)
  • Viscometry
  • Conductometry
  • Microcalorimetry
  • Spectroscopic analysis (UV-Vis, CD)

Related Experiment Videos

  • Viscometric measurements

    • Main Results:

    • At low CTAB concentrations, adsorption to pepsin enhances hydrophobicity and lowers interfacial tension.
    • Formation of a polymer-surfactant coacervate observed.
    • CTAB interaction leads to pepsin unfolding and altered interfacial tension profiles.
    • Discrepancies in aggregation behavior observed across different techniques (tensiometry, viscometry vs. conductometry, microcalorimetry).
    • Microcalorimetry confirmed CTAB's extended critical micelle concentration (cmce) in the presence of pepsin.

    Conclusions:

    • Pepsin-CTAB interactions result in complex formation and micelle generation with unique properties.
    • CTAB significantly influences pepsin's structural integrity and interfacial behavior.
    • The study highlights the utility of multiple techniques for comprehensive interaction analysis.