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The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro

Stephen A Newland1, Iain C Macaulay, Andres R Floto

  • 1Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Addembrookes Hospital, Hills Road, Cambridge, UK.

Blood
|February 22, 2007
PubMed

The G6B cell-surface receptor, which contains a single Ig-like domain, has been shown to bind to SHP-1 and SHP-2 after phosphorylation of 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail, classifying this protein as a new member of the family of inhibitory receptors. In this study, we demonstrate by real-time polymerase chain reaction (PCR) and Western-blot analysis that G6B is expressed on platelets. Cross-linking of G6B with polyclonal antisera has a significant inhibitory effect on platelet aggregation and activation by agonists such as ADP and collagen-related peptide (CRP). This inhibitory function of G6B appears to operate in a calcium-independent manner. Our results suggest that G6B represents a novel inhibitory receptor found on the surface of platelets and that it could be an antithrombotic drug target.

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