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Updated: Jul 16, 2026
04:25
CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
Published on: June 13, 2025
Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors
Kazuhiro Chiba1, Yasutaka Hoshino, Keiko Ishino
1Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Japanese Journal of Infectious Diseases
|February 23, 2007
Abstract:
We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
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