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Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging
Hwan Myung Kim1, Pil Rye Yang, Mun Sik Seo
1Molecular Opto-Electronics Laboratory, Department of Chemistry and Center for Electro- and Photo-Responsive Molecules, Graduate School of Life Sciences and Biotechnology, Korea University, 1-Anamdong, Seoul 136-701, Korea.
Abstract:
A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).
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