Related Experiment Video
Updated: Jul 16, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
Published on: June 23, 2022
Metal particle-enhanced fluorescent immunoassays on metal mirrors
Evgenia G Matveeva1, Ignacy Gryczynski, Anne Barnett
1Department of Molecular Biology and Immunology, Health Science Center, University of North Texas, Fort Worth, TX 76107, USA. ematveev@hsc.unt.edu <ematveev@hsc.unt.edu>
Abstract:
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.
More Related Videos
Related Concept Videos
Immunogold Electron Microscopy
Immunofluorescence Microscopy
The...

