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Updated: Jul 16, 2026

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
Published on: March 1, 2019
In vitro sildenafil citrate use as a sperm motility stimulant
1Andrology & Sexology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. taymour1155@link.net
Abstract:
The effect of different concentrations of sildenafil citrate (Viagra) solution in vitro on human sperm motility was assessed in 85 asthenozoospermic semen specimens. Semen samples were divided equally into six tubes, one as a control and to the others, sildenafil dissolved solution was added (v/v) in vitro with five different concentrations (4.0, 2.0, 1.0, 0.5, 0.1 mg/mL). The tubes were incubated and were followed up for sperm motility changes at 0.5, 1, 2, and 3 hours. It is demonstrated that the in vitro use of sildenafil citrate solution has a concentration-related stimulatory effect on ejaculated sperm motility.
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