Preparation and study on the solid inclusion complex of cloxacillin sodium with beta-cyclodextrin

Area of Science:

• Pharmaceutical Chemistry
• Supramolecular Chemistry
• Analytical Chemistry

Background:

• Cloxacillin sodium is a semi-synthetic penicillin antibiotic.
• Beta-cyclodextrin (beta-CD) is a cyclic oligosaccharide known for its ability to form inclusion complexes.
• Understanding drug-excipient interactions is crucial for drug formulation and delivery.

Purpose of the Study:

• To synthesize and characterize a solid inclusion complex of cloxacillin sodium with beta-cyclodextrin.
• To investigate the interaction between cloxacillin sodium and beta-CD using various analytical techniques.
• To determine the stoichiometry and formation constant of the cloxacillin-beta-CD complex.

Main Methods:

• Coprecipitation method for synthesizing the inclusion complex.
• Spectroscopic techniques: fluorescence spectroscopy and infrared (IR) spectroscopy.
• Nuclear Magnetic Resonance (NMR): 1D and 2D NMR (including 1H NMR) for structural elucidation and complex characterization.

Main Results:

• Successful synthesis of a solid inclusion complex between cloxacillin sodium and beta-CD.
• Characterization data from fluorimetry, IR, and NMR confirmed the formation of the inclusion complex.
• Experimental evidence indicated a 1:1 stoichiometry for the cloxacillin sodium-beta-CD complex.
• The formation constant of the complex was determined using fluorescence and 1H NMR methods.
• 2D NMR techniques provided insights into the spatial configuration of the complex.

Conclusions:

• A stable 1:1 inclusion complex of cloxacillin sodium with beta-cyclodextrin was successfully synthesized and characterized.
• The study provides a comprehensive understanding of the cloxacillin sodium-beta-CD interaction, relevant for pharmaceutical applications.
• The proposed spatial configuration offers valuable information for designing advanced drug delivery systems involving cyclodextrins.