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Updated: Jul 16, 2026

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
Published on: March 1, 2011
PC12 cells express IL-1 receptor type I and response to IL-1beta stimulation
Hai-Feng Shu1, Bai-Ren Wang, Hui Bi
1Institute of Neurosciences, The Fourth Military Medical University, Xi'an 710032, PR China.
Abstract:
PC12 cell line has been widely used in a diverse array of neurophysiological studies including in exploration of oxygen-sensing mechanism. In present study, we first identified with immunocytochemistry and Western blot methods that interleukin-1 receptor type I was expressed in the PC12 cells. We then demonstrated with patch clamping technique that extracellular application of IL-1beta dose-dependently inhibited the outward voltage-dependent and TEA-sensitive potassium currents (I(K)) in the PC12 cells, and pre-incubation with the interleukin-1 receptor antagonist almost completely abolished this inhibitory effect. In addition, application of IL-1beta shifted steady-state inactivation of I(K) in hyperpolarizing direction, but did not alter its steady-state activation. Furthermore, IL-1beta-induced inhibition of I(K) led to a membrane depolarization and a transient increase of [Ca(2+)](i) in PC12 cells. Taking together, the present study elucidates that PC12 cells bear interleukin-1 receptor and response to IL-1beta stimulation.
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