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Updated: Jul 16, 2026
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
Published on: April 4, 2016
Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA
B A P Buscher1, H Gerritsen, I van Schöll
1Business Unit Analytical Research, TNO Quality of Life, P.O. Box 360, 3700 AJ Zeist, The Netherlands. brigitte.buscher@tno.nl
Abstract:
An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; M(W) 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats.
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