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Replication of the R6K plasmid during the Escherichia coli cell cycle
J D Keasling1, B O Palsson, S Cooper
1Department of Chemical Engineering, University of Michigan, Ann Arbor 48109-2136.
Journal of Bacteriology
|February 1, 1992
Abstract:
The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner.