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Updated: Jul 16, 2026
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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Diagnostic potential for urinary proteomics
Glen L Hortin1, Denis Sviridov
1National Institutes of Health, Department of Laboratory Medicine, Warren Magnuson Clinical Center, Building 10, Room 2C-407, Bethesda, MD 20892-1508, USA. ghortin@mail.cc.nih.gov
Pharmacogenomics
|February 28, 2007
Summary
Urine analysis offers a promising, non-invasive method for diagnosing kidney diseases and systemic conditions. Advanced proteomic techniques reveal over 1000 proteins in urine, enhancing diagnostic potential for conditions like transplant rejection.
Area of Science:
- Biochemistry
- Clinical Diagnostics
- Proteomics
Background:
- Urine is a plasma ultrafiltrate with significantly lower protein concentrations than plasma.
- Despite low protein levels, urine collection is non-invasive and many urinary protein tests are clinically established.
- Proteomic technologies have identified over 1000 proteins and peptides in urine, expanding its diagnostic utility.
Purpose of the Study:
- To highlight the diagnostic potential of urine as a specimen for identifying kidney and systemic diseases.
- To discuss the advantages and challenges of using urine in clinical diagnostics.
- To showcase the role of proteomics in expanding the analysis of urinary proteins.
Main Methods:
- Analysis of urinary protein composition using proteomic technologies.
- Comparison of urine and plasma protein profiles.
- Review of established and emerging urinary protein biomarkers.
Main Results:
- Urine contains a wide array of plasma proteins, with a higher proportion of low-molecular-weight proteins and peptides.
- Over 1000 distinct proteins and peptides have been identified in urine.
- Urine analysis can be enriched for proteins originating from the urinary tract.
Conclusions:
- Urine, despite challenges, holds significant potential as a diagnostic specimen for both systemic and renal diseases.
- Proteomics enhances the ability to detect disease-specific urinary protein signatures.
- Applications include sensitive detection of kidney transplant rejection and medication-induced renal toxicity.
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