[Diversity and quantity of different microorganisms in methanogenic granular sludge]

PCR-DGGE, FISH and RTQ-PCR techniques were used to study the microbial community structure and quantity of different microorganisms in the methanogenic granule in different phases from a lab-scale anaerobic reactor. The results indicated that as the organic loading rate of the reactor increasing, the archaea community changed more significantly than bacterial community. Most bacteria were located in the out layer of granule, while most archaea were located in the inner layer. The quantity of archaea was a little less than bacteria, and the quantity of methanosaeta increased significantly.