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Structure determination in situ by averaging of tomograms

Friedrich Förster1, Reiner Hegerl

  • 1Department of Pharmaceutical Sciences, University of California, San Francisco, California 94143, USA.

Methods in Cell Biology
|March 1, 2007
PubMed
Abstract

No abstract available in PubMed .

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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