Related Experiment Video
Updated: Jul 16, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
Validated MALDI-TOF/TOF mass spectra for protein standards
Jayson A Falkner1, Maureen Kachman, Donna M Veine
1National Resource for Proteomics and Pathway, Michigan Proteome Consortium, Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.
Abstract:
A current focus of proteomics research is the establishment of acceptable confidence measures in the assignment of protein identifications in an unknown sample. Development of new algorithmic approaches would greatly benefit from a standard reference set of spectra for known proteins for the purpose of testing and training. Here we describe an openly available library of mass spectra generated on an ABI 4700 MALDI TOF/TOF from 246 known, individually purified and trypsin-digested protein samples. The initial full release of the Aurum Dataset includes gel images, peak lists, spectra, search result files, decoy database analysis files, FASTA file of protein sequences, manual curation, and summary pages describing protein coverage and peptides matched by MS/MS followed by decoy database analysis using Mascot, Sequest, and X!Tandem. The data are publicly available for use at ProteomeCommons.org.
More Related Videos
Related Concept Videos
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry
Matrix-Assisted Laser Desorption Ionization (MALDI)
Rapid Identification of Pathogens
Mass Spectrometry: Overview

