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Updated: Jul 16, 2026

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
Published on: April 26, 2018
Detection of biomarkers for alcoholism by two-dimensional differential gel electrophoresis
Di Wu1, Takeshi Tomonaga, Kazuyuki Sogawa
1Department of Molecular Diagnosis (F8), Graduate School of Medicine, Chiba University, Chiba, Japan.
Background:
Up to now, gamma-glutamyltransferase (gamma-GTP) and carbohydrate-deficient transferrin (CDT) have been used as markers for alcoholism most widely, but they are not satisfactory regarding sensitivity/specificity. Therefore, for novel markers need to be searched.
Methods:
To detect new biomarkers for alcoholism, albumin and immunoglobulinG were first removed from serum. Then, protein profiles of 12 serum samples before and after 3 months of abstinence treatment were examined using agarose 2-dimensional differential gel electrophoresis (agarose2-D DIGE). Two-dimensional differential gel electrophoresis images were analyzed using Shimadzu 2-D Evolution Software.
Results:
Eight spots whose expression were significantly altered after abstinence were detected. Of these, 2 proteins increased and 6 proteins decreased after treatment.
Conclusions:
Altered expressions of several serum proteins after abstinence therapy were detected. They are promising markers for clinical application of alcoholism.
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