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Adsorption Isotherms I01:29

Adsorption Isotherms I

Adsorption isotherms are mathematical models that describe how molecules in a gas or liquid phase interact with surfaces. Two of the most common isotherm models are the Langmuir and Freundlich isotherms, which relate to Type I monolayer chemisorption. The Langmuir model is based on four key assumptions:• Adsorption cannot exceed monolayer coverage.• All surface sites are equivalent.• Molecules adsorb only at vacant sites.• There are no interactions between adsorbed molecules.Consider the...
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Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies
07:31

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Published on: September 1, 2023

Modelling and simulation of affinity membrane adsorption.

Cristiana Boi1, Simone Dimartino, Giulio C Sarti

  • 1DICMA, Università di Bologna, viale Risorgimento 2, 40136 Bologna, Italy. cristiana.boi@mail.ing.unibo.it

Journal of Chromatography. A
|March 3, 2007
PubMed
Summary

A new mathematical model describes biomolecule adsorption on affinity membranes, incorporating diffusion, kinetics, and flow dynamics. This model accurately predicts human IgG adsorption, aiding large-scale membrane adsorber process assessment.

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Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Mathematical Modeling

Background:

  • Affinity membranes are crucial for biomolecule purification.
  • Accurate modeling is needed to optimize large-scale membrane adsorber processes.
  • Understanding adsorption kinetics and fluid dynamics is key.

Purpose of the Study:

  • To develop a comprehensive mathematical model for biomolecule adsorption on affinity membranes.
  • To incorporate convection, diffusion, adsorption kinetics, dead volumes, and flow distribution.
  • To validate the model using experimental data for human IgG adsorption.

Main Methods:

  • Developed a mathematical model integrating transport phenomena and adsorption kinetics.
  • Obtained simulation parameters from equilibrium and dynamic experimental data.
  • Identified a bi-Langmuir kinetic mechanism for human IgG adsorption.
  • Simulated breakthrough curves and compared them with experimental data.

Main Results:

  • The model accurately describes convection, diffusion, and adsorption kinetics.
  • Bi-Langmuir kinetics were identified as crucial for process description.
  • Simulated breakthrough curves showed good agreement with experimental data.
  • The model accounts for dead volumes and lag times.

Conclusions:

  • The proposed model offers new insights into affinity membrane adsorption phenomena.
  • It is a valuable tool for assessing membrane adsorbers in large-scale applications.
  • Accurate kinetic mechanism identification is vital for process simulation.