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Updated: Jul 16, 2026

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease
Published on: February 8, 2022
Tricolored "tricolore" myocardium by selective intracoronary injection of contrast using 256-slice cone-beam computed
Abstract:
We report our experience with 256-slice cone-beam computed tomography following selective coronary arterial bolus injection in pigs, which distinguished the segmented left ventricular (LV) myocardium supplied by each coronary artery into three parts more clearly than with other modalities. Two pigs were anesthetized and catheters positioned in the left anterior descending branch (LAD) of the coronary artery in pig 1 and the left circumflex branch (LCx) in pig 2. 10 ml of iodinated contrast material diluted with 40 ml of saline was injected at a rate of 3 ml/s. Entire heart scanning was started simultaneously and continued for 25 s. We selected the most static images of the LV at around 5 s after contrast injection. Axial source and multiplanar reconstruction images from the right anterior oblique projection clearly revealed tricolored, segmented LV myocardial enhancement of the anterior and apical walls and inter-ventricular septum in pig 1, and the lateral and posterior walls in pig 2. We were able to identify the borders between the LV myocardium supplied by the LAD, the LCx and the right coronary artery, respectively, and this technique may facilitate new cardiovascular diagnoses.
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