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Related Experiment Video

Updated: Jul 16, 2026

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
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Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

Published on: April 21, 2022

Mesothelin expression in human lung cancer.

Mitchell Ho1, Tapan K Bera, Mark C Willingham

  • 1Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|March 3, 2007
PubMed
Summary

Mesothelin is frequently found on lung adenocarcinoma cells, making it a promising target for immunotherapy. The SS1P immunotoxin shows significant cytotoxicity against these cancer cells.

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Area of Science:

  • Oncology
  • Immunotherapy
  • Molecular Biology

Background:

  • Mesothelin is a cell surface glycoprotein implicated in various cancers.
  • Identifying novel therapeutic targets is crucial for improving lung cancer treatment outcomes.

Purpose of the Study:

  • To evaluate mesothelin as a potential therapeutic target for lung cancer immunotherapy.
  • To assess the expression of mesothelin in lung cancer tissues and cell lines.

Main Methods:

  • Mesothelin mRNA and protein expression analyzed using RT-PCR, immunoblotting, and immunohistochemistry.
  • Cell surface mesothelin expression quantified by flow cytometry.
  • Cytotoxic activity of mesothelin-specific immunotoxin SS1P evaluated against lung cancer cell lines.

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Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice
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Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Published on: December 21, 2019

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Last Updated: Jul 16, 2026

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
08:01

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

Published on: April 21, 2022

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice
07:54

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Published on: December 21, 2019

Main Results:

  • Mesothelin mRNA detected in 83% of lung adenocarcinomas and 78% of lung cancer cell lines.
  • High mesothelin protein expression observed on the surface of lung adenocarcinoma cells and specific cell lines (NCI-H322M, EKVX).
  • Immunotoxin SS1P demonstrated potent cytotoxic effects on mesothelin-expressing lung cancer cells (IC50: 2-5 ng/mL).

Conclusions:

  • Mesothelin is highly expressed on the surface of most lung adenocarcinoma cells.
  • The mesothelin-specific immunotoxin SS1P exhibits significant cytotoxicity against relevant lung cancer cell lines.
  • Mesothelin represents a promising therapeutic target for non-small cell lung cancer, warranting further clinical evaluation.