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Updated: Jul 16, 2026

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
Published on: May 28, 2007
In situ hybridization of termite microbes
Shigeharu Moriya1, Satoko Noda, Moriya Ohkuma
1Environmental Molecular Biology Laboratory, RIKEN Institute, Yokohama City, Japan.
Abstract:
In situ hybridization is one of the most direct and reliable ways to ascertain the origin of the gene from complex mixed cellular systems. This method is essential for studying communities of uncultured microorganism in their natural ecosystem. In this chapter, we introduce our protocols for the in situ hybridization of the messenger RNA of uncultured symbiotic protists of termite hindgut and the ribosomal RNA of the symbiotic bacteria of the protists using nonradioactive labeling protocols. We hope that you will find these protocols useful in your own work to unravel the complex functions and to discover new organisms in the ecosystem.
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