Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MediLabSecure: A One Health Network Facing the COVID-19 Pandemic DVM.

Health security·2026
Same author

Development of a qPCR molecular diagnostic assay for the detection of kiwi Eimeria species and its application to determine tissue-specificity of species causing coccidiosis in North Island brown kiwi (Apteryx mantelli).

Parasitology research·2025
Same author

Contrasting levels of transcriptome-wide SNP diversity and adaptive molecular variation among conifers.

Frontiers in plant science·2025
Same author

Emergence and spread of the SARS-CoV-2 omicron (BA.1) variant across Africa: an observational study.

The Lancet. Global health·2025
Same author

De novo transcriptome assembly and discovery of drought-responsive genes in white spruce (Picea glauca).

PloS one·2025
Same author

Detection of Bacterial Infections and Malaria among Blood Culture-Negative Samples of Hospitalized Febrile Patients from a Tertiary Hospital in Ethiopia.

The American journal of tropical medicine and hygiene·2024

Related Experiment Video

Updated: Jan 9, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

5.2K

Real-time PCR fluorescent chemistries.

John Mackay1, Olfert Landt

  • 1Applied Science, Roche Diagnostics, Mt Wellington, Auckland, New Zealand.

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2007
PubMed
Summary

Explore diverse fluorescent detection chemistries for real-time PCR, including popular options like SYBR Green I and TaqMan probes. Newer dyes and probe formats offer enhanced DNA detection and multiplexing capabilities for accurate quantification and genotyping.

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Kinetic (real-time) PCR relies on fluorescent detection of amplified DNA.
  • Multiple detection chemistries exist, with SYBR Green I, TaqMan, and hybridization probes being widely used.
  • Standard chemistries may not always provide optimal performance or features.

Purpose of the Study:

  • To review available fluorescent detection chemistries for real-time PCR.
  • To highlight the benefits of alternative chemistries.
  • To introduce emerging dyes and probe formats for improved performance.

Main Methods:

  • Literature review of fluorescent detection methods in real-time PCR.
  • Comparison of commonly used chemistries (SYBR Green I, TaqMan, hybridization probes).
  • Discussion of novel dyes and probe formats.

More Related Videos

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

17.8K
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

15.9K

Related Experiment Videos

Last Updated: Jan 9, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

5.2K
qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

17.8K
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

15.9K

Main Results:

  • Over a dozen fluorescent detection formats are available for real-time PCR.
  • Many chemistries are adaptable across different real-time PCR instruments.
  • Newer dyes offer superior fluorescent detection and product resolution.
  • Novel probe formats enable advanced multiplexing for quantification and genotyping.

Conclusions:

  • Alternative fluorescent chemistries can offer advantages over instrument-specific recommendations.
  • Emerging technologies in fluorescent detection promise enhanced capabilities for DNA analysis.
  • Continued innovation in real-time PCR chemistries supports advancements in molecular diagnostics and research.