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05:46
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
Published on: June 9, 2020
Glomerulus-specific mRNA transcripts and proteins identified through kidney expressed sequence tag database analysis
L He1, Y Sun, J Patrakka
1Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Kidney International
|March 3, 2007
Summary
Researchers identified 497 glomerulus-enriched gene transcripts, including novel candidates, by comparing mouse glomerular and whole kidney expressed sequence tag (EST) profiles. Further study of these kidney filter genes will advance understanding of glomerular development and function.
Area of Science:
- Nephrology
- Molecular Biology
- Genomics
Background:
- The kidney glomerulus is vital for blood filtration, but its molecular makeup and function remain incompletely understood.
- Previous work involved constructing and sequencing mouse glomerular expressed sequence tag (EST) libraries from newborn and adult samples.
Purpose of the Study:
- To identify glomerulus-enriched transcripts by comparing glomerular EST profiles with whole kidney EST profiles.
- To discover novel genes and proteins involved in glomerular development and physiology.
Main Methods:
- Expressed Sequence Tag (EST) library sequencing and comparative analysis.
- In situ hybridization for RNA expression localization.
- Human Protein Atlas verification for protein expression.
- Taqman quantitative polymerase chain reaction for mRNA abundance validation.
Main Results:
- Identified 497 glomerulus-enriched transcripts (UniGene clusters) with higher expression in glomeruli than in whole kidney.
- Confirmed expression of known glomerulus-specific genes.
- Discovered numerous novel gene transcripts potentially involved in glomerular functions.
- Validated glomerulus-specific RNA expression for six novel genes and protein expression for two others.
Conclusions:
- The study identified a significant number of novel glomerulus-enriched genes.
- Further characterization of these identified genes and proteins is expected to enhance the understanding of glomerular development and physiology.
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