Related Experiment Video
Updated: Jan 26, 2026
08:32
Author Spotlight: Advancing Male Infertility Research by Unraveling Sperm Metabolism and Mitochondrial Function
Published on: June 23, 2023
2.9K
Expression of various CDC25B isoforms in human spermatozoa
Yen-Ni Teng1, Chia-Ling Chung, Yung-Ming Lin
1Department of Biotechnology, Chia Nan University of Pharmacy and Science, National Cheng Kung University, College of Medicine, Tainan, Taiwan.
Fertility and Sterility
|March 6, 2007
Summary
CDC25B protein variants are expressed in human sperm, with altered levels in asthenospermia. This suggests diverse roles beyond cell division in mature male germ cells.
Area of Science:
- Reproductive genetics
- Molecular biology
- Spermatogenesis
Background:
- CDC25B protein is a phosphatase involved in cell cycle regulation.
- Its role in terminally differentiated cells like spermatozoa is not well understood.
Purpose of the Study:
- To investigate the expression and potential functions of CDC25B in human spermatozoa.
- To determine if CDC25B expression is altered in cases of male infertility.
Main Methods:
- Utilized reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR to analyze CDC25B mRNA.
- Employed immunostaining and immunofluorescence to assess CDC25B protein localization.
- Examined sperm samples from fertile and infertile volunteers.
Main Results:
- Identified four splicing variants of CDC25B (CDC25B1-4) in human spermatozoa.
- Localized CDC25B protein primarily to the midpiece, with weaker signals in the principal piece and sperm head cytosol.
- Observed increased CDC25B mRNA levels in men with asthenospermia (reduced sperm motility).
Conclusions:
- The presence and localization of CDC25B in spermatozoa indicate roles beyond cell-cycle control.
- CDC25B may have specific functions in mature human germ cells, potentially related to sperm function or survival.
- Aberrant CDC25B expression could be linked to male infertility conditions like asthenospermia.
Related Concept Videos
01:42What is Gene Expression?
195.2K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.2K
01:36What is Gene Expression?
11.2K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.2K
01:58Cell Specific Gene Expression
16.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
02:35Chromatin Position Affects Gene Expression
24.7K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.7K
02:48Expressing Solution Concentration
66.2K
A solute is a component of a solution that is typically present at a much lower concentration than the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.
66.2K
01:26Emotional Expression
999
Emotional expression encompasses how individuals convey their emotions through verbal communication and non-verbal cues. These non-verbal actions include facial expressions, body language, and physical gestures, such as frowning or smiling. Among these, facial expressions play a crucial role in emotional expression and are understood universally, indicating a biological basis for how humans communicate emotions.
Universal Facial Expressions
Psychologist Paul Ekman identified seven basic...
Universal Facial Expressions
Psychologist Paul Ekman identified seven basic...
999