Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Functional Diversity of Two Novel Embryonic Microglial Subpopulations and Their Developmental Trajectories in Developing Mouse Brains.

Glia·2025

Same author

Blastocyst telomere length predicts successful implantation after frozen-thawed embryo transfer.

Human reproduction open·2024

Same author

TIAM2S-positive microglia enhance inflammation and neurotoxicity through soluble ICAM-1-mediated immune priming.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2023

Same author

Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis.

Science advances·2023

Same author

Development and validation of an expanded targeted sequencing panel for non-invasive prenatal diagnosis of sporadic skeletal dysplasia.

BMC medical genomics·2021

Same author

TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.

Cancers·2020

Same journal

Correction to "Long-Read Sequencing of CAH and ADPKD Provides Novel Insights Into the Genetic Diagnosis of Male Infertility".

Reproductive medicine and biology·2026

Same journal

The Impact of Leukemia Inhibitory Factor on <i>Sirtuin1</i>, <i>Ilt-4</i>, and Related microRNAs Expression in a Mouse Model of Recurrent Pregnancy Loss.

Reproductive medicine and biology·2026

Same journal

Rethinking Oocyte Yield in Advanced Maternal Age: Toward Age- and Biology-Calibrated Counseling Targets in IVF.

Reproductive medicine and biology·2026

Same journal

International Expert Consensus Statement on Chronic Endometritis: A Comprehensive Literature Review and Modified e-Delphi Study.

Reproductive medicine and biology·2026

Same journal

The Role of Vitamins in Preconception Care and Infertility Treatment: A Narrative Review.

Reproductive medicine and biology·2026

Same journal

Association Between Hysteroscopic Treatment of Cesarean Scar Disorder and Changes in the Endometrial Microbiome and Clinical Outcomes: A Prospective Observational Study.

Reproductive medicine and biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 14, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Optimizing Non-Invasive PGT-A: A Multi-Factorial Approach for Enhanced Accuracy and Seamless Integration Into

Hsien-An Pan^1,2, Yen-An Tang^3,4, I-Ning Huang³

¹An-An Women and Children Clinic Tainan Taiwan.

Reproductive Medicine and Biology

|October 20, 2025

Summary

This summary is machine-generated.

Non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) offers accurate chromosomal assessment, improving IVF outcomes. This optimized workflow enhances embryo selection and reduces invasive procedures for better reproductive medicine.

Keywords:

assisted hatching meta‐analysis non‐invasive preimplantation genetic testing for aneuploidy (niPGT‐A)ongoing pregnancy rate spent culture media

More Related Videos

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

FISH for Pre-implantation Genetic Diagnosis

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

Related Experiment Videos

Last Updated: Jan 14, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

FISH for Pre-implantation Genetic Diagnosis

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

Area of Science:

Reproductive Medicine
Genetics
Embryology

Background:

Preimplantation genetic testing for aneuploidy (PGT-A) is crucial for in vitro fertilization (IVF) success.
Traditional PGT-A involves invasive trophectoderm (TE) biopsy, posing risks to embryo development.
Non-invasive PGT-A (niPGT-A) using spent culture medium (SCM) offers a less invasive alternative.

Purpose of the Study:

To identify factors influencing niPGT-A accuracy and effectiveness.
To develop clinical integration workflows for niPGT-A.
To provide alternative embryo assessment and improve pregnancy outcomes in IVF.

Main Methods:

A two-phase study analyzed 341 blastocysts and SCM from 90 IVF patients.
Phase 1: Assessed niPGT-A accuracy by comparing SCM/TE with whole blastocysts (100 embryos).
Phase 2: Examined concordance, assisted hatching, and culture duration effects on outcomes after frozen embryo transfer (241 embryos); included meta-analysis.

Main Results:

Optimized niPGT-A workflow achieved 92.1% PPV and 91.3% accuracy, outperforming conventional PGT-A.
Ploidy and sex concordances were 76.5% and 98.9% for fresh embryos.
SCM-guided embryo transfer significantly improved pregnancy outcomes compared to TE biopsy in meta-analysis.

Conclusions:

Optimized niPGT-A provides accurate chromosomal assessment and integrates into IVF workflows.
It supports informed embryo transfer decisions, improving outcomes and reducing invasive procedure risks.
This method enhances clinical management in reproductive medicine by offering a less invasive approach.