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Related Experiment Videos

Actin is involved in auxin-dependent patterning.

Jan Maisch1, Peter Nick

  • 1Institute of Botany 1, University of Karlsruhe, D-76128 Karlsruhe, Germany. jan.maisch@botanik1.uni-karlsruhe.de

Plant Physiology
|March 6, 2007
PubMed
Summary
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This study reveals that auxin influences its own transport by altering actin filament organization. Disrupting actin bundling in tobacco cells affects cell division synchrony and auxin transport sensitivity.

Area of Science:

  • Plant biology
  • Cell biology
  • Biochemistry

Background:

  • Polar auxin transport is crucial for plant pattern formation.
  • Auxin transport polarity is linked to PIN-formed protein cycling and actomyosin-dependent vesicle traffic.
  • The role of actin in regulating auxin transport remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of actin in regulating auxin transport and cell division synchrony.
  • To understand how auxin influences its own transport dynamics.

Main Methods:

  • Utilized patterned cell division in tobacco BY-2 cells to monitor auxin flux polarity.
  • Manipulated actin organization by overexpressing mouse talin to induce actin bundling.
  • Assessed the impact of actin bundling on cell division synchrony and sensitivity to auxin transport inhibitors.

Related Experiment Videos

  • Investigated the effect of specific auxins on actin organization and cell division.

    • Main Results:

    • Cell division in tobacco BY-2 cells exhibits partial synchrony, which can be disrupted by inhibiting auxin transport.
    • Overexpression of mouse talin to bundle actin impaired cell division synchrony and increased sensitivity to 1-N-naphthylphthalamic acid.
    • Indole-3-acetic acid and 1-naphthyl acetic acid, but not 2,4-dichlorophenoxyacetic acid, restored normal actin organization and cell division synchrony.

    Conclusions:

    • Auxin appears to regulate its own transport by modulating the state of actin filaments.
    • Actin organization plays a significant role in the synchrony of cell division and auxin transport.
    • Specific auxins can rescue actin organization and cell division synchrony, suggesting a feedback mechanism.