Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interlaboratory performance testing on EPIC v2.0 CNS tumor profiling demonstrates high reproducibility of tumor classification but reveals the need for harmonized copy number variation reporting.

Brain pathology (Zurich, Switzerland)·2026
Same author

The effect of different milk pretreatment methods on microbiome community development during Herrgårds cheese production and ripening.

PloS one·2026
Same author

A comprehensive reference genome assembly dataset of birds inhabiting Denmark, Greenland, and the Faroe Islands.

GigaScience·2026
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Temporal genomics reveal rapid adaptation to pesticide exposure in Eastern honeybees.

National science review·2026
Same author

Paleogenomes reveal the evolutionary relationship between modern and cave lions.

Cell·2026

Related Experiment Video

Updated: Feb 17, 2026

An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions
07:44

An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions

Published on: November 17, 2023

13.4K

DNA extraction from dry museum beetles without conferring external morphological damage.

M Thomas P Gilbert1, Wendy Moore, Linea Melchior

  • 1Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America. mtpgilbert@gmail.com

Plos One
|March 8, 2007
PubMed
Summary

Researchers developed a new DNA extraction method for dry insects, preserving specimen integrity. This technique allows genetic analysis of valuable specimens, including rare and type specimens, without causing external damage.

More Related Videos

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

7.2K
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
10:09

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Published on: February 3, 2020

19.6K

Related Experiment Videos

Last Updated: Feb 17, 2026

An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions
07:44

An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions

Published on: November 17, 2023

13.4K
A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

7.2K
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
10:09

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Published on: February 3, 2020

19.6K

Area of Science:

  • Entomology
  • Molecular Biology
  • Genetics

Background:

  • Vast collections of dry-preserved insects offer potential for genetic research.
  • Previous DNA recovery methods caused specimen destruction, limiting studies on rare or type specimens.

Purpose of the Study:

  • To develop a non-destructive DNA extraction method for dry-preserved insects.
  • To enable genetic analysis of valuable insect specimens without external morphological damage.

Main Methods:

  • Developed a novel protocol for extracting DNA from dry insects.
  • Utilized Polymerase Chain Reaction (PCR) to amplify mitochondrial (cytochrome c oxidase I) and nuclear (28s ribosomal DNA) gene fragments.
  • Validated the method on beetle specimens up to 50 years old.

Main Results:

  • Successfully extracted PCR-amplifiable mitochondrial and nuclear DNA from dry insect specimens.
  • Demonstrated that the method does not cause external morphological damage to the specimens.
  • Confirmed the efficacy of the DNA extraction on aged specimens.

Conclusions:

  • The described method provides a valuable tool for genetic research on dry-preserved insects.
  • Enables the study of genetic material from rare and type specimens without compromising their morphological integrity.
  • Facilitates broader genetic analyses from existing entomological collections.