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Updated: Jul 16, 2026

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Published on: March 12, 2019
[Influence of glucose-6-phosphate dehydrogenase on rat adrenal 11-beta-hydroxylase]
R Lauro1, D Fonzo, L Dogliotti
1Istituto di Patologia Speciale Medica e Metodologia Clinica dell'Università di Torino.
Abstract:
Previous work has shown NADPH to be a necessary cofactor in adrenal production of steroids. Synthesis is, in effect, a function of dehydrogenase activity and the adrenals are particularly rich in these enzymes. The present paper deals with an appraisal of the importance of G 6-PD as a source of NADPH for mitochondrial hydroxylation. 11-beta-hydroxylation was used as parameter. It was found that the activity of this enzyme, and hence NAPDH production, was stimulated by the administration of ACTH; no parallel increase in 11-beta-hydroxylase activity was noted.
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