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Mouse podocyte complement factor H: the functional analog to human complement receptor 1.

Jessy J Alexander1, Ying Wang, Anthony Chang

  • 1Section of Nephrology, University of Chicago, 5841 S. Maryland Avenue, MC5100, Chicago, IL 60637, USA.

Journal of the American Society of Nephrology : JASN
|March 9, 2007
PubMed
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Complement factor H (Cfh) is crucial for kidney health, protecting against glomerular disease. Locally produced Cfh in podocytes limits complement activation and immune complex processing, acting as a vital surrogate for human complement receptor 1.

Area of Science:

  • Immunology
  • Nephrology
  • Complement System Biology

Background:

  • Complement factor H (Cfh) regulates the alternative complement pathway.
  • Cfh has roles beyond plasma, including on platelets and podocytes.
  • Podocyte Cfh may function analogously to human erythrocyte complement receptor 1.

Purpose of the Study:

  • To investigate the role of Cfh in the kidney, specifically in glomerulonephritis.
  • To determine the impact of local versus plasma Cfh on kidney disease outcomes.
  • To explore podocyte-derived Cfh function in immune complex processing.

Main Methods:

  • Renal transplantation between wild-type (WT) and Cfh-deficient (Cfh(-/-)) mice.
  • Induction of chronic serum sickness via apoferritin immunization.

Related Experiment Videos

  • Assessment of renal function (albuminuria, BUN) and glomerular pathology (immune deposits).

    • Main Results:

    • WT kidneys in Cfh(-/-) recipients developed glomerulonephritis, unlike in WT recipients.
    • Cfh(-/-) kidneys in WT recipients showed increased albuminuria and BUN, with subendothelial/subepithelial IgG and C3 deposits.
    • WT kidneys in Cfh(-/-) hosts had podocyte-associated Cfh and reduced IgG deposits, suggesting local Cfh limits immune complex deposition.

    Conclusions:

    • Plasma Cfh is essential for preventing glomerulonephritis in this model.
    • Locally produced podocyte Cfh is critical for processing immune complexes in the subepithelial space.
    • Podocyte Cfh acts as a functional surrogate for human complement receptor 1 in the kidney.