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Related Experiment Videos

Rayon balls and disposable-diaper material selectively adsorb creatinine.

D M Mock1

  • 1Department of Pediatrics, University of Iowa, Iowa City 52242.

The American Journal of Clinical Nutrition
|February 1, 1992
PubMed
Summary

Infant urine collection using diaper materials can alter creatinine concentration. Rayon balls and diaper material significantly decrease creatinine levels, impacting sample accuracy.

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Area of Science:

  • Clinical Chemistry
  • Biochemical Analysis
  • Pediatric Medicine

Background:

  • Urine sample collection in infants often uses absorbent materials in diapers.
  • Creatinine concentration is a key biomarker used to normalize urine excretion measurements.
  • The accuracy of creatinine normalization depends on the integrity of the urine sample post-collection.

Purpose of the Study:

  • To investigate the impact of absorbent materials on urinary creatinine concentration.
  • To determine if urine absorption by cotton, rayon, or diaper material introduces artifacts.
  • To assess the reliability of using creatinine concentration from absorbed infant urine samples.

Main Methods:

  • Urine samples from adults and infants were tested.
  • Creatinine concentration was measured before and after absorption by cotton balls, rayon balls, and diaper material.
  • Experiments were conducted across a range of sample wetness.

Main Results:

  • Rayon balls and diaper material significantly decreased creatinine concentration in urine samples.
  • This artifactual decrease was more pronounced in lightly wetted samples.
  • Cotton balls showed a smaller, less significant decrease in creatinine concentration (3% vs. 9-13% for rayon/diaper material).

Conclusions:

  • Rayon balls and diaper material selectively adsorb creatinine from human urine.
  • These materials can introduce significant artifacts into infant urine sample analysis.
  • The use of rayon balls and diaper material for urine collection may compromise the accuracy of creatinine-based measurements.

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