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Using PCR to extend the limit of oligonucleotide synthesis
M L Michaels1, H M Hsiao, J H Miller
1Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
Biotechniques
|January 1, 1992
Abstract:
A method has been developed to allow one to extend the practical limit of oligonucleotide synthesis by coupling the synthesis reaction to a subsequent PCR. Given that DNA synthesizers are capable of producing reasonable yields of oligonucleotides that are 125-150 bases in length, this method could be used to recover the minute amount of full-length product present in mixtures extended well beyond the established limits. This technology could be applied to gene synthesis and mutagenesis.