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Recognition of tertiary structure in tRNAs by Rh(phen)2phi3+, a new reagent for RNA structure-function mapping

C S Chow1, L S Behlen, O C Uhlenbeck

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.

Biochemistry
|February 4, 1992
PubMed
Summary

Rhodium complex Rh(phen)2phi3+ selectively cleaves tRNA at tertiary interaction sites, revealing structural insights. This photoactivated reagent targets accessible major grooves, aiding in probing RNA structural changes and validating tRNA tertiary structure.

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