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Related Experiment Videos

Plastid DNA from Pyrenomonas salina (Cryptophyceae): physical map, genes, and evolutionary implications.

M Maerz1, J Wolters, C J Hofmann

  • 1Institut für Biologie II, Lehrstuhl für Zellbiologie, Freiburg, Federal Republic of Germany.

Current Genetics
|January 1, 1992
PubMed
Summary

Cryptomonad plastid DNA, originating from red algae symbiosis, was mapped and sequenced. Phylogenetic analysis confirms cryptomonad plastids

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Area of Science:

  • Algal genomics
  • Molecular evolution
  • Symbiosis research

Background:

  • Cryptomonads likely evolved from a eukaryotic host and an algal symbiont related to red algae.
  • Organellar DNA is crucial for understanding phylogenetic relationships.

Purpose of the Study:

  • To characterize the plastid (pt) DNA of the cryptomonad alga Pyrenomonas salina.
  • To elucidate the phylogenetic position of cryptomonad plastids.

Main Methods:

  • Restriction mapping of 127 kb circular ptDNA.
  • Localization of ten protein genes and rRNA genes via hybridization and sequence homology.
  • Sequencing of the plastidal small subunit (SSUr) RNA gene.
  • Phylogenetic analysis using the neighborliness method.

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Main Results:

  • A restriction map of Pyrenomonas salina ptDNA was established, featuring an inverted repeat (IR) region and two single-copy regions.
  • Genes for rRNA, key proteins (e.g., RuBisCO, photosystem II, ATP synthase), and ribosomal proteins were localized.
  • Phylogenetic analysis of the SSUr gene indicates a close relationship between cryptomonad plastids and red algae.

Conclusions:

  • The study provides a detailed characterization of Pyrenomonas salina plastid DNA.
  • Phylogenetic data strongly supports the red algal origin of cryptomonad plastids.