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Author Spotlight: Studying Behavior of Acanthamoeba to Develop Targeted Strategies for Preventing Acanthamoeba Keratitis
Published on: September 20, 2024
Characteristics of motive force derived from trajectory analysis of Amoeba proteus
Noritaka Masaki1, Hiromi Miyoshi, Yoshimi Tsuchiya
1Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midoriku, Yokohama, Japan.
Abstract:
We used a monochromatic charge-coupled-device camera to observe the migration behavior of Amoeba proteus every 5 s over a time course of 10000 s in order to investigate the characteristics of its centroid movement (cell velocity) over the long term. Fourier transformation of the time series of the cell velocity revealed that its power spectrum exhibits a Lorentz type profile with a relaxation time of a few hundred seconds. Moreover, some sharp peaks were found in the power spectrum, where the ratios of any two frequencies corresponding to the peaks were expressed as simple rational numbers. Analysis of the trajectory using a Langevin equation showed that the power spectrum reflects characteristics of the cell's motive force. These results suggest that some phenomena relating to the cell's motility, such as protoplasmic streaming and the sol-gel transformation of actin filaments, which seem to be independent phenomena and have different relaxation times, interact with each other and cooperatively participate in the generation process of the motive force.
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