Related Experiment Video
Updated: Jul 16, 2026

Murine Nasal Lavage Fluid Collection without Blood Contamination
Published on: July 11, 2025
Risk of contamination of nasal sprays in otolaryngologic practice
Erdinc Aydin1, Evren Hizal, Babur Akkuzu
1Department of Otorhinolaryngology, Baskent University Faculty of Medicine, Ankara, Turkey. erdinca@baskent-ank.edu.tr
Background:
Reusable nasal-spray devices are frequently used in otolaryngologic examinations, and there is an increasing concern about the risk of cross-contamination from these devices. The aim of our study was to determine, by means of microbiologic analysis, the safety of a positive-displacement or pump-type atomizer after multiple uses.
Methods:
A reusable nasal spray bottle, pump, and tips were used in the nasal physical examination of 282 patients admitted to a tertiary otolaryngology clinic. The effectiveness of 2 different methods of prophylaxis against microbiologic contamination (the use of protective punched caps or rinsing the bottle tip with alcohol) was compared with that of a control procedure.
Results:
Although there was no statistically significant difference in positive culture rates among the types of nasal spray bottles tested, methicillin-resistant coagulase-negative staphylococci were isolated in 4 of 198 cultures.
Conclusion:
Given these findings, we concluded that additional precautions (such as the use of an autoclave between sprays, disposable tips, or disposable devices) are warranted to avoid interpatient cross-contamination from a reusable nasal spray device.
Insights
Reusable nasal spray devices pose cross-contamination risks. Microbiologic analysis revealed methicillin-resistant coagulase-negative staphylococci in cultures, indicating a need for enhanced safety measures in otolaryngology.
Area of Science:
- Otolaryngology
- Microbiology
- Infection Control
Background:
- Reusable nasal spray devices are common in otolaryngologic exams.
- Concerns exist regarding cross-contamination risks associated with these devices.
- This study investigated the safety of pump-type atomizers after repeated use.
Purpose of the Study:
- To assess the microbiologic safety of reusable positive-displacement nasal spray devices.
- To evaluate the effectiveness of prophylactic measures against contamination.
Main Methods:
- Microbiologic analysis of a reusable nasal spray bottle, pump, and tips used on 282 patients.
- Comparison of two prophylaxis methods (protective caps, alcohol rinsing) against a control.
- Culture analysis to detect microbial contamination.
Main Results:
- No statistically significant difference in positive culture rates between tested nasal spray bottle types.
- Isolation of methicillin-resistant coagulase-negative staphylococci in 4 out of 198 cultures.
- Detection of potentially pathogenic bacteria indicates a risk of transmission.
Conclusions:
- Standard prophylactic measures were insufficient to prevent microbial contamination.
- Additional precautions are necessary to avoid interpatient cross-contamination.
- Recommendations include autoclaving, disposable tips, or disposable devices.
More Related Videos
09:03Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic
Published on: November 7, 2020
05:43Topical Airway Anesthesia for Awake-endoscopic Intubation Using the Spray-as-you-go Technique with High Oxygen Flow
Published on: January 13, 2017
Related Concept Videos
Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
Suctioning the Nasopharyngeal Airway
Equipment Required
Transmission-based Precautions I: Contact, Enteric, and Droplets
Contact Precautions:
Contact precautions are the measures taken to prevent the transmission of infectious agents, especially epidemiologically important microorganisms such as MRSA or influenza, primarily transmitted through direct or indirect contact with an...
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Epistaxis
Etiology
Possible causes of this condition include high blood pressure, trauma, low humidity, upper respiratory tract infections, allergies, foreign bodies, nasal inhalation of corticosteroids or illicit drugs, excessive use of decongestant nasal sprays, facial or nasal surgery, anatomic malformation, tumors, or systemic...
Suctioning the Oropharyngeal Airway
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...