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Related Experiment Videos

Bioluminescent assays for high-throughput screening.

Frank Fan1, Keith V Wood

  • 1Promega Corporation, Madison, WI 53711, USA. frank.fan@promega.com

Assay and Drug Development Technologies
|March 16, 2007
PubMed
Summary
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Luminescent assays offer superior sensitivity and robustness for high-throughput screening (HTS) in drug discovery. Recent advancements in engineered luciferase systems enhance detection capabilities for various biological targets.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Fluorescence assays dominate high-throughput screening (HTS), but luminescence assays are gaining traction.
  • Luminescence offers advantages like high sensitivity, broad linearity, and robustness in complex biological samples.

Purpose of the Study:

  • To review recent advancements in bioluminescent assays for HTS.
  • To highlight new detection capabilities from engineered luciferase genes, enzymes, and substrates.

Main Methods:

  • Engineering luciferase genes for improved expression efficiency and reduced lifetime in reporter assays.
  • Modifying luciferase enzymes for enhanced stability in ATP and luciferin detection assays.
  • Developing synthetic luciferin derivatives for specific enzyme detection.

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Main Results:

  • Enhanced sensitivity and response dynamics in genetic reporter assays.
  • Improved stability and performance of ATP and luciferin detection assays.
  • Emergence of intracellular biosensors for dynamic physiological process monitoring.

Conclusions:

  • Bioluminescent assays are increasingly valuable for HTS due to technological advancements.
  • Engineered luciferase systems provide versatile and sensitive detection methods.
  • Future applications include dynamic monitoring of cellular processes via novel biosensors.