[Effects of keratinocyte growth factor on the expression and activity of extracellular signal-regulated kinase in Hep-2 cell line]
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Updated: Jun 26, 2026

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Published on: May 26, 2017
Rong Fang1, Jin Hu, Hongtao Zhen
1Department of Otolaryngology, the First People's Hospital of Yichang, Yichang, 443003, China.
Objective:
To observe effects of keratinocyte growth factor (KGF) on the proliferation of Hep-2 cell line; the activity and expression of extracellular signal-regulated kinase (ERK).
Method:
Cell line proliferation was measured by Flow cytometry and MTT assay; ERK activity was measured by ERKl/2 immuno-precipitation kinase assay kit; and p ERK expression was assessed by Western blot.
Result:
KGF can improve the proliferation of Hep-2 cell line. The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF.
Conclusion:
KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.
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