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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Direct imaging of lipid-ion network formation under physiological conditions by frequency modulation atomic force
Takeshi Fukuma1, Michael J Higgins, Suzanne P Jarvis
1Centre for Research on Adaptive Nanostructures and Nanodevices, Lincoln Place Gate, Trinity College Dublin, Dublin 2, Ireland.
Abstract:
Various metal cations in physiological solutions interact with lipid headgroups in biological membranes, having an impact on their structure and stability, yet little is known about the molecular-scale dynamics of the lipid-ion interactions. Here we directly investigate the extensive lipid-ion interaction networks and their transient formation between headgroups in a dipalmitoylphosphatidylcholine bilayer under physiological conditions. The spatial distribution of ion occupancy is imaged in real space by frequency modulation atomic force microscopy with sub-Angstrom resolution.
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