Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Network activity-independent coordinated gene expression program for synapse assembly.

Luis M Valor¹, Paul Charlesworth, Lawrence Humphreys

¹Genes to Cognition Programme, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America

|March 16, 2007

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Region- and layer-specific glutamatergic synapse development in the nascent cortical hierarchy.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same author

Overview of transcriptomics and epigenomics approaches in the diagnosis, prognosis, and therapeutics of primary brain cancers.

Epigenomics·2026

Same author

Single-cell transcriptomics and mouse model phenotyping for biomarker screen of peripheral blood in Huntington's disease.

Scientific reports·2026

Same author

PRMix: Primary Region Mix Augmentation and Benchmark Dataset for Precise Whole Mouse Brain Anatomical Delineation.

NeuroImage·2026

Same author

Protein trafficking and synaptic demand configure complex and dynamic synaptome architectures of individual neurons.

Scientific reports·2026

Same author

Synapse types are spatially associated with regional hemodynamics in the mouse brain.

PLoS biology·2026

Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

Two sequential gene expression programs drive synapse development and function. The first program governs synapse assembly, preceding neuronal activity, while the second regulates activity-dependent gene expression for network maturation.

Area of Science:

Neuroscience
Genomics
Molecular Biology

Background:

Genomics, transcriptomics, and proteomics offer novel insights into genome-synapse interactions.
Understanding synapse formation and function requires integrating genetic and neuronal activity data.

Purpose of the Study:

To investigate the genomic programs underlying synapse formation and activity-dependent gene regulation.
To elucidate the temporal relationship between gene expression, protein synthesis, and neuronal network activity during synaptogenesis.

Main Methods:

Combined transcriptome analysis and multielectrode recordings in mouse embryonic primary neuronal cultures.
Analysis of gene expression patterns related to postsynaptic and plasticity proteins.

Main Results:

Related Experiment Videos

Identified a coordinated gene expression program involving 642 genes for synapse assembly.
Observed that this synaptogenesis program precedes synapse marker protein expression and neuronal spiking.
Demonstrated sequential gene expression programs: synaptogenesis, followed by activity-dependent gene expression during network maturation.

Conclusions:

Two distinct, sequentially activated gene expression programs are fundamental to synaptic function and neuronal network development.
Gene expression plays a critical, time-ordered role in orchestrating synapse formation, maturation, and activity-dependent plasticity.