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Updated: Jul 16, 2026
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Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
[Construction of Sjcb2 DNA vaccine and its expression in HeLa cells]
Yong-xuan Hu1, Jian-hua Xiao, Jia-fang Huang
1Institute of Pathogenic Biology, Nanhua University, Hengyang 421001, China.
Abstract:
A recombinant plasmid containing cathepsin B endopeptidase of Schistosoma japonicum (Sjcb2) was constructed, indentified by PCR, restrictive enzyme, digestion and DNA sequencing, and expressed into mammalian cells. Immunochemistry examination showed that the Sjcb2 gene can be expressed in the eukaryotic system, providing a basis for the development of schistosome DNA vaccine.
