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Updated: Jul 16, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents
Babu Lal Somani1, Vivek Ambade
1Department of Biochemistry, Armed Forces Medical College, Pune-411040, India. blsomani@gmail.com
Objectives:
To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.
Design And Methods:
Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.
Results:
Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively.
Conclusions:
Norfloxacin method is a simple, inexpensive and automatable kinetic assay.
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