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[Evaluation of two techniques of molecular subtyping to study Pasteurella multocida]

G A Leotta1, I Chinen, G B Vigo

  • 1Servicio Fisiopatogenia,Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas--Administracidó Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrin, Buenos Aires, Argentina. galeotta@anlis.gov.ar

Revista Argentina De Microbiologia
|March 22, 2007
PubMed

Insights

Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) and ApaI-Pulsed Field Gel Electrophoresis (Apal-PFGE) effectively determine genetic relationships in P. multocida strains. Combining both methods enhances epidemiological studies by accurately grouping related strains and differentiating unrelated ones.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Epidemiology

Background:

  • Pasteurella multocida (P. multocida) is an important pathogen with diverse strains.
  • Accurate methods are needed to establish genetic relatedness for epidemiological investigations.

Purpose of the Study:

  • To evaluate the typeability, reproducibility, and discriminatory power of ERIC-PCR and Apal-PFGE for P. multocida strain typing.
  • To assess the utility of these techniques, individually and in combination, for epidemiological studies.

Main Methods:

  • Forty-nine P. multocida strains from various sources were analyzed using ERIC-PCR and Apal-PFGE.
  • ERIC-PCR generated 31 patterns with 10-14 bands (0.2-1.2 kb).
  • Apal-PFGE generated 37 patterns with 7-15 bands (34-450 kb).

Main Results:

  • Both methods demonstrated 100% reproducibility.
  • ERIC-PCR had 100% typeability and 93% discriminatory power.
  • Apal-PFGE had 94% typeability and 98% discriminatory power.
  • Combined use of both techniques effectively grouped related strains and differentiated unrelated ones.

Conclusions:

  • ERIC-PCR and Apal-PFGE are reliable and powerful tools for P. multocida strain typing.
  • The combination of ERIC-PCR and Apal-PFGE significantly enhances epidemiological investigations of P. multocida infections.
  • These methods are valuable complements for understanding P. multocida transmission and outbreaks.

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