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Related Experiment Videos

Changes in erythrocyte membrane lipid composition affect the transient decrease in membrane order which accompanies

M T Santini1, R Masella, A Cantafora

  • 1Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.

Experientia
|January 15, 1992
PubMed
Summary
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Altered erythrocyte membranes with higher cholesterol content inhibit insulin receptor internalization. Increased membrane fluidity, not order, is necessary for insulin receptor down-regulation.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Membrane Biophysics

Background:

  • Insulin receptor internalization (down-regulation) in human erythrocytes involves a transient decrease in membrane order.
  • Membrane lipids significantly influence receptor internalization processes.

Purpose of the Study:

  • To investigate the impact of altered lipid profiles, specifically increased cholesterol, on insulin receptor down-regulation.
  • To determine if changes in membrane order are linked to altered lipid composition in erythrocytes.

Main Methods:

  • Utilized electron paramagnetic resonance (EPR) spectroscopy with a 5-nitroxystearate spin label to measure membrane order (2T' parallel order parameter).
  • Examined human erythrocytes enriched with cholesterol and erythrocytes from cirrhotic patients (higher cholesterol/phospholipid ratio).

Related Experiment Videos

  • Assessed insulin receptor down-regulation using binding assays over a 3-hour incubation at 37°C.

    • Main Results:

    • Cholesterol-enriched and cirrhotic erythrocytes showed no significant insulin receptor down-regulation.
    • These erythrocytes also failed to exhibit the transient decrease in membrane order observed in control cells.
    • A higher cholesterol/phospholipid molar ratio correlated with a lack of down-regulation and membrane fluidity changes.

    Conclusions:

    • A more ordered erythrocyte membrane, due to increased cholesterol, inhibits insulin receptor internalization.
    • Increased membrane disorder appears to be a necessary prerequisite for insulin receptor down-regulation in erythrocytes.