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Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
Masaru Ihira1, Shiho Akimoto, Fumi Miyake
1Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan.
Background:
A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories.
Objectives:
We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera.
Results:
The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively.
Conclusion:
Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).
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